ABSTRACT

   Typhoid fever (TF) is an acute systemic infection caused by Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B. It is transmitted by the fecal oral route mainly via contaminated food and water. The developing countries have high rate of morbidity and mortality due to Typhoid fever, epidemics take place in developed world also. There are increased incidences of multi drug resistant in S. typhi strains that has further complicated its management and only a few antibiotics are now effective in treatment of typhoid. We report that the aqueous extracts of fruit peel Citrus sinensis (L.) confer anti typhoid activity against Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B respectively on comparison with ciprofloxacin.



        

Key words:
Typhoid fever, Salmonella Typhi, Salmonella Para Typhi A , Salmonella Para Typhi B, anti typhoid activity, Citrus sinensis.
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ABSTRACT
  
 
A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method employing electrospray ionization was developed and validated for the simultaneous determination of hydrochlorothiazide and bisoprolol in human plasma. the analytes and the internal standard moxifloxacin were separated on a Hypersil, hypurity C8 column (125mm4mm i.d. 5m particle size) analytical column from Thermo Electron Corporation (Chesshire, U.K.). The mobile phase was an ammonium acetate solution (1.5 mM) with formic acid (0.1%): methanol and acetonitrile (60:20:20 v/v/v (%)), the flow rate of the mobile phase under isocratic condition was kept at 0.500 mL/min. Sample preparationwas performed by protein precipitation with acetonitrile, Quantitation was done on a triple quadruple mass analyzer API - 3000, equipped with turbo ion spray interface and operating in Multiple Reaction Monitoring (MRM) mode. Linearity in plasma was demonstrated over the concentration range1.00-80.00 ng/mL for hydrochlorothiazide and 0.10-30.0 (ng/mL) for bisoprolol. The method was validated for Selectivity and specificity, Recovery, Linearity, Precision and accuracy, Bench-top stability, Freeze-thaw stability, autosampler stability and Long term stability in matrix. This method showed good performances in terms of The intra- and inter-day precision was within (2.50 -7.50%) and accuracy (-0.58 - 10.03%) , The mean extraction recoveries were 95.60%, 36%, for both bisoprolol and hydrochlorothiazide respectively


Key words:
 
LC-ESI-MS/MS, hydrochlorothiazide, bisoprolol and electrospray ionization

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May 2011 Issue
May - 2011 / Volume - 3/Issue - 03
( Total Articles =25 )
Arjun Singh
Article No 3
May - 2011 / Volume - 3 / Issue - 03 / Article No - 3 / Research Article

HIGH THROUGHPUT LIQUID CHROMATOGRAPHY IONSPRAY TANDEM MASS SPECTROMETRY (LC-ESI-MS/MS) METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS QUANTIFICATION OF HYDROCHLOROTHIAZIDE AND BISOPROLOL IN HUMAN PLASMA.

Arjun Singh1,2*, Dr.T.Sivakumar2, Dr. D.A.Jain1,
Umesh mishra1, Arun Kumar yadav2 ,Tarun Sharma4

1Bhagwant university (Pharmacy dept.)
Sikar road Ajmer Rajasthan 305004 India.
2Glenmark Research centre.  MIDC,
Mahape, Navi Mumbai-400709, India
                                                                                                                                                              
E-mail: arjunssingh7@gmail.com