Typhoid fever (TF) is an acute systemic infection caused by Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B. It is transmitted by the fecal oral route mainly via contaminated food and water. The developing countries have high rate of morbidity and mortality due to Typhoid fever, epidemics take place in developed world also. There are increased incidences of multi drug resistant in S. typhi strains that has further complicated its management and only a few antibiotics are now effective in treatment of typhoid. We report that the aqueous extracts of fruit peel Citrus sinensis (L.) confer anti typhoid activity against Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B respectively on comparison with ciprofloxacin.
Key words: Typhoid fever, Salmonella Typhi, Salmonella Para Typhi A , Salmonella Para Typhi B, anti typhoid activity, Citrus sinensis.
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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
Screening and isolation of proteolytic bacteria was carried out from the soil samples collected from the surroundings of University College of Technology,Osmania University,Hyderabad.And identified as Bacillus subtilis SVR-07.The Bacillus subtilis SVR-07 could be grow up to 60oC with in a broad pH range of 6.0 to 11.0 with an optimal growth temperature and pH at 55oC and 9.0 respectively.Different optimization parameters like Incubation period(48hrs), Inoculum level(3ml),Age of inoculum(24h),Effect of inhibitors(AgNO3) and different types of surfactants(Cetrimide) on alkaline protease production by using isolated Bacillus subtilis SVR-07.The results showed that the Bacillus subtilis SVR-07 under study are good producer of extra cellular protease at high temperature.This might be an indication that proteases produced would be thermo stable.
Key words: Alkaline protease, Bacillus subtilis SVR-07, Submerged fermentation, Optimization
March - 2011 / Volume - 3/Issue - 1
( Total Articles =25 )
March 2011 Issue
Article No 21
March - 2011 / Volume - 3 / Issue - 01 / Article No - 21 / Research Article
EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM ISOLATED BACILLUS SUBTILIS SVR-07 BY USING SUBMERGED FERMENTATION
C. Ganesh Kumar1 K.Swathi2, B.Nagamani2,
S. Venkateshwar2, L.Venkateswar Rao3
1Chemical Biology,Indian Institute of Chemical Technology,
Hyderabad, India-500 007
2Centre for biotechnology, University College of Technology,
Osmania university, Hyderabad, 500 007,
Andhra Pradesh, India
3Department of Microbiology, Osmania university,
Hyderabad, 500 007, Andhra Pradesh, India
M. Narsi Reddy