Typhoid fever (TF) is an acute systemic infection caused by Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B. It is transmitted by the fecal oral route mainly via contaminated food and water. The developing countries have high rate of morbidity and mortality due to Typhoid fever, epidemics take place in developed world also. There are increased incidences of multi drug resistant in S. typhi strains that has further complicated its management and only a few antibiotics are now effective in treatment of typhoid. We report that the aqueous extracts of fruit peel Citrus sinensis (L.) confer anti typhoid activity against Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B respectively on comparison with ciprofloxacin.
Key words: Typhoid fever, Salmonella Typhi, Salmonella Para Typhi A , Salmonella Para Typhi B, anti typhoid activity, Citrus sinensis.
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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
An isocratic reverse phase liquid chromatography (RP-LC) method has been developed and subsequently validated for the determination of Entecavir in pharmaceutical formulation. Separation was achieved with a Develosil ODS MG-5 (250 mmx4.6 mm I.D; particle size 5 µm) and Trifluoroacetic acid Buffer (0.5 ml in 2000ml of water v/v): Acetonitrile (93:7) as eluent at flow rate 1.0 mL/min. UV detection was performed at 254nm. The method is simple, rapid, and selective. The described method of Entecavir is linear over a range of 4.8 µg/mL to 71.93 µg/mL. The method precision for the determination of assay was below 2.0%RSD. The percentage recoveries of active pharmaceutical ingredient (API) from dosage forms ranged from 99.5 to 100.2. The method is useful in the quality control of pharmaceutical formulations.
Key words: LC Determination, Entecavir
Article No 25
February - 2011 / Volume - 2 / Issue - 12 / Article No - 25 / Research Article
LC DETERMINATION OF ENTECAVIR IN PHARMACEUTICAL FORMULATION
1Department of Pharmaceutical Sciences,
Andhra University, Visakhapatnam-530 003, India
V. Kalyana C.
February - 2011 / Volume - 2/Issue - 12
( Total Articles =25 )
February 2011 Issue