ABSTRACT

   Typhoid fever (TF) is an acute systemic infection caused by Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B. It is transmitted by the fecal oral route mainly via contaminated food and water. The developing countries have high rate of morbidity and mortality due to Typhoid fever, epidemics take place in developed world also. There are increased incidences of multi drug resistant in S. typhi strains that has further complicated its management and only a few antibiotics are now effective in treatment of typhoid. We report that the aqueous extracts of fruit peel Citrus sinensis (L.) confer anti typhoid activity against Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B respectively on comparison with ciprofloxacin.



        

Key words:
Typhoid fever, Salmonella Typhi, Salmonella Para Typhi A , Salmonella Para Typhi B, anti typhoid activity, Citrus sinensis.
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January - 2010 / Volume - 2/Issue - 11
( Total Articles =15 )
January 2011 Issue
ABSTRACT

This has been proven experimentally that DNA is the molecule of heredity. Gel electrophoresis is a method that separates macromolecules such as nucleic acids or proteins. The electrophoresis term is used to describe the migration of charged particle under the influence of an electric field. Thus gel electrophoresis refers is the technique in which molecules are forced across a span of gel, motivated by an electrical current. On either end of the gel there are activated electrodes that provide the driving force. Therefore a molecule's properties especially the possession of ionisable groups, determine how rapidly an electric field can move the molecule through a gelatinous medium. One very important application for gel electrophoresis is in DNA Technology. We are now using biotechnology to study the basic processes of life, diagnose illnesses, and develop new treatments for diseases. For example, isolation of DNA from animals, vegetables and microorganisms. Isolated DNA molecule from onion for enhancing gene expression of a coding sequence, fragment, genetic variant, cassette, vector, cell, plant and seed.



Key words:
DNA, Gel Electrophoresis, Onion
Article No 5
January - 2011 / Volume - 2 / Issue - 11 / Article No - 5 / Research Article

A BIOTECHNOLOGICAL SIGNIFICANCE: ISOLATION OF DNA FROM ONION


Kapil K. Kulkarni
1*, Rajesh J Oswal1,
Rishikesh V Antre1, Deepak T Panjwani1


1Pharmaceutical Biotechnology Research Lab,
JSPM’s Charak college of Pharmacy and Research,
Wagholi, Pune, Maharashtra, India.
Email: jspmpharmacy@gmail.com 
Kapil Kulkarni
Only 15 Articles will be Published in Each Issues