Typhoid fever (TF) is an acute systemic infection caused by Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B. It is transmitted by the fecal oral route mainly via contaminated food and water. The developing countries have high rate of morbidity and mortality due to Typhoid fever, epidemics take place in developed world also. There are increased incidences of multi drug resistant in S. typhi strains that has further complicated its management and only a few antibiotics are now effective in treatment of typhoid. We report that the aqueous extracts of fruit peel Citrus sinensis (L.) confer anti typhoid activity against Salmonella Typhi, Salmonella Para Typhi A and Salmonella Para Typhi B respectively on comparison with ciprofloxacin.
Key words: Typhoid fever, Salmonella Typhi, Salmonella Para Typhi A , Salmonella Para Typhi B, anti typhoid activity, Citrus sinensis.
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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
December - 2010 / Volume - 2/Issue - 10
( Total Articles =17 )
December 2010 Issue
Article No 12
December - 2010 / Volume - 2 / Issue - 10 / Article No - 10 / Research Article
STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMBROXOL HYDROCHLORIDE AND ROXITHROMYCIN IN BULK AND TABLET DOSAGE FORM
Dhiraj S.Nikam1*, Swapnil C.Aswale1
1Department of Pharmaceutical Chemistry,
School of Pharmacy Technology Management,
Babulde, Shirpur, Dhule, M.S., India 425 405
Dhiraj S. NIkam
A stability indicating reverse phase high performance liquid chromatographic method is developed for simultaneous estimation of ambroxol hydrochloride and roxithromycin in bulk and pharmaceutical formulation.The separation is made by phenomenex Gemini C18 column (250 cm × 4.6 mm, 5 µm) using water: acetonitrile: orthophosphoric acid (50:50:0.1) as a mobile phase. Detection is carried out at 210 nm. The retention time of ambroxol hydrochloride and roxithromycin is 2.33 and 4.22 min, respectively. The validation of the method is performed according to International Conference on Harmonization ICH Q2B guideline. Specificity, reproducibility, precision and accuracy are confirmed. The limits of quantification are approximately 0.75 µg/ml for ambroxol hydrochloride and 1.93 µg/ml for roxithromycin. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.
Key words: RP-HPLC, ambroxol hydrochloride, Roxithromycin,stability-indicating.