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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
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ABSTRACT

A simple reverse phase HPLC method has been developed and validated for the simultaneous determination of prazosin hydrochloride and three calcium channel blockers; diltiazem hydrochloride, verapamil and amlodipine besylate in bulk material, their finished product and human serum. The separation was carried out using a mobile phase consisting of acetonitrile:methanol:water: (10:55:35 v/v, pH 2.650.02), on a Nuclosil 100-10 C-18 (2504.6mm), 10 micron particle size column. Studies were performed on a HPLC system equipped with a UV/visible detector at 240 nm. The flow rate employed for analysis was 1mL min-1 in isocratic mode. All drugs were eluted within 6 minutes and give sharp peak with high theoretical plate count and low tailing factor. The retention time of prazosin, verapamil, diltiazem and amlodipine were 3.02, 3.84, 3.94 and 4.92 minutes respectively. The validation study was carried out according to ICH guide lines. The described method was linear over a concentration range of 5-100 g mL-1, 10-200 g mL-1, 15-300 g mL-1 and 30-600 g mL-1 for the assay of prazosin, diltiazem, amlodipine and verapamil respectively. The limit of quantitation and the limit of detection were found to be 0.0994, 0.0926, 0.1642, 0.0905 g mL-1 and 0.0328, 0.0306, 0.0542 and 0.0299 g mL-1 for prazosin, amlodipine, diltiazem and verapamil respectively. Therefore, the study showed that the proposed RP-HPLC method is rapid, simple, specific, precise and accurate, which is useful for the routine determination of prazosin, amlodipine, diltiazem and verapamil in bulk materials, dosage formulations and serum.


Key words:
Prazosin hydrochloride, verapamil, diltiazem hydrochloride, amlodipine, method validation.

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November - 2010 / Volume - 2/Issue - 9
( Total Articles =31 )
November 2010 Issue
Article No 2
November - 2010 / Volume - 2 / Issue - 9 / Article No -2 / Review Article

SIMULTANEOUS DETERMINATION OF PRAZOSIN AND CALCIUM CHANEL BLOCKERS IN RAW MATERIALS, PHARMACEUTICAL FORMULATIONS AND HUMAN SERUM BY RP-HPLC

  Shabana Naz1,*
Najma Sultana1,
M. Saeed Arayne2,Nighat Shafi1

1Research Institute of Pharmaceutical Sciences,
Faculty of Pharmacy, University of Karachi,
Karachi-75270, Pakistan.
1Department of Chemistry, University of Karachi.
Karachi-75270, Pakistan.
Email:Shabana.naz.shah@gmail.com
Shabana Naz
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