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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
Copyright 2009  by "International Journal of Pharmaceutica Research and Development"    All Rights reserved    E-Mail: info@ijprd.com
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Online Since : March 2009
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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND CONFIRMATION OF SECONDARY METABOLITES BY HPTLC FINGERPRINTING METHOD OF SOME IMPORTANT PLANT SPECIES OF GENUS SIDA


Rajesh R. Wake1*,
Sharad N. Shinde1, Narhari A. Patil1, Umakant K. Halde1

1
Principal, Lokseva College of Pharmacy,
Phulgaon, Pune-412216, MH, India


May - 2013 / Volume - 5/Issue - 3
( Total Articles =20)
May 2013 Issue
ABSTRACT
The study represents preliminary phytochemical analysis and HPTLC fingerprinting of extracts of three ethnomedicinally important plant species of genus Sida viz., Sida cordifolia, Sida rhombifolia and Sida acuta (Malvaceae). Phytochemical analysis of these extracts revealed the presence of secondary metabolites such as alkaloids, tannins, flavonoids, steroids, saponins and glycosides. HPTLC fingerprinting for flavonoids in ethanolic extract of S. acuta gave good fingerprints with major and sharp peak at Rf 0.30. Ethanolic extract of all 3 plants showed peaks at Rf 0.57, 0.56 and 0.30 and are present in good yields in both S. cordifolia and S. acuta. For tannins, S. cordifolia showed good fingerprint results for all extracts with major peaks at Rf 0.72, 0.78, 0.77, 0.78 and 0.34. Petroleum ether and benzene extracts of S. acuta showed good fingerprint with major peaks at Rf 0.75 and 0.78, while chloroform, acetone and ethanol extracts did not show many peaks.

KEYWORDS : Phytochemical, HPTLC, ethnomedicine, fingerprinting, Sida etc.


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