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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
Copyright 2009  by "International Journal of Pharmaceutica Research and Development"    All Rights reserved    E-Mail: info@ijprd.com
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Online Since : March 2009
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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND DEVELOPMENT
VALIDATED HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF QUERCETIN AND KAEMPFEROL IN METHANOLIC EXTRACT OF TRIDAX PROCUMBENS

Saraswathy Nagendran1*,

Vahbiz Patel1, Nidhi Sharma1, Jay Savai1, Meena Chintamaneni1

1
Shobhaben Pratabhai Patel School of Pharmacy & Technology Management,NMIMS, Mumbai, India.
May - 2013 / Volume - 5/Issue - 3
( Total Articles =20)
May 2013 Issue
ABSTRACT
A rapid and sensitive High-performance thin layer chromatography (HPTLC) method was developed and validated for the simultaneous determination of Quercetin and Kaempferol in the methanolic extract of Tridax procumbens plant. Tridax procumbens plant was collected from Dhule District, Shirpur, India and comparative study was performed for presence of standards - Quercetin and Kaempferol using HPTLC technique. HPTLC of flavonoids was performed on F254 TLC plates with Toluene: Ethyl acetate: Methanol: Formic acid (6:4:0.5:0.2) as mobile phase. Densitometric determination of flavonoids was performed at
? = 254 nm in reflectance/absorbance mode. The linear regression analysis data for the calibration plots showed a good linear relationship in the concentration range of 100-1000ng with r2= 0.997 for Quercetin and r2= 0.906 for Kaempferol with respect to peak area. The recovery for Quercetin was 98.60% and for Kaempferol was 90.08% indicating accuracy of method. HPTLC method analyzed; represented an excellent technique for simultaneous determination of Quercetin and Kaempferol in the methanolic extract of Tridax procumbens plant, with good sensitivity, selectivity, precision and accuracy.

KEYWORDS :
:  Tridax Procumbens, Quercetin, Kaempferol, Flavonoids, HPTLC, Accuracy, Recovery, Sensitivity.

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